Engineering of flavonoid O-methyltransferase for a novel regioselectivity.
Identifieur interne : 003277 ( Main/Exploration ); précédent : 003276; suivant : 003278Engineering of flavonoid O-methyltransferase for a novel regioselectivity.
Auteurs : Eun Ji Joe [Corée du Sud] ; Bong-Gyu Kim ; Byoung-Chan An ; Youhoon Chong ; Joong-Hoon AhnSource :
- Molecules and cells [ 0219-1032 ] ; 2010.
Descripteurs français
- KwdFr :
- Biotransformation (MeSH), Chromatographie en phase liquide à haute performance (MeSH), Escherichia coli (métabolisme), Flavonoïdes (métabolisme), Ingénierie des protéines (méthodes), Kaempférols (analyse), Kaempférols (composition chimique), Methyltransferases (composition chimique), Methyltransferases (métabolisme), Modèles moléculaires (MeSH), Populus (enzymologie), Protéines mutantes (composition chimique), Protéines mutantes (métabolisme), Protéines recombinantes (isolement et purification), Quercétine (analyse), Quercétine (composition chimique), Réaction de polymérisation en chaîne (MeSH), Sites de fixation (MeSH), Spécificité du substrat (MeSH), Stéréoisomérie (MeSH).
- MESH :
- analyse : Kaempférols, Quercétine.
- composition chimique : Kaempférols, Methyltransferases, Protéines mutantes, Quercétine.
- enzymologie : Populus.
- isolement et purification : Protéines recombinantes.
- métabolisme : Escherichia coli, Flavonoïdes, Methyltransferases, Protéines mutantes.
- méthodes : Ingénierie des protéines.
- Biotransformation, Chromatographie en phase liquide à haute performance, Modèles moléculaires, Réaction de polymérisation en chaîne, Sites de fixation, Spécificité du substrat, Stéréoisomérie.
English descriptors
- KwdEn :
- Binding Sites (MeSH), Biotransformation (MeSH), Chromatography, High Pressure Liquid (MeSH), Escherichia coli (metabolism), Flavonoids (metabolism), Kaempferols (analysis), Kaempferols (chemistry), Methyltransferases (chemistry), Methyltransferases (metabolism), Models, Molecular (MeSH), Mutant Proteins (chemistry), Mutant Proteins (metabolism), Polymerase Chain Reaction (MeSH), Populus (enzymology), Protein Engineering (methods), Quercetin (analysis), Quercetin (chemistry), Recombinant Proteins (isolation & purification), Stereoisomerism (MeSH), Substrate Specificity (MeSH).
- MESH :
- chemical , analysis : Kaempferols, Quercetin.
- chemical , chemistry : Kaempferols, Methyltransferases, Mutant Proteins, Quercetin.
- chemical , isolation & purification : Recombinant Proteins.
- chemical , metabolism : Flavonoids, Methyltransferases, Mutant Proteins.
- enzymology : Populus.
- metabolism : Escherichia coli.
- methods : Protein Engineering.
- Binding Sites, Biotransformation, Chromatography, High Pressure Liquid, Models, Molecular, Polymerase Chain Reaction, Stereoisomerism, Substrate Specificity.
Abstract
An O-methyltransferase isolated from poplar, POMT7, was identified as a flavone 7-O-methyltransferase. In order to generate a mutant of POMT-7 having a novel regioselectivity, we conducted an error-prone polymerase chain reaction. More than 100 mutants were screened and one of the mutants (POMT-M1) Asp257Gly, methylated the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The mutation changed asparagine residue at the position of 257 into glycine. The kinetic parameters showed that the wild type POMT7 was better activity toward kaempferol and quercetin than the POMT7-M1. Using E. coli transformant expressing POMT7-M1, 58 microM of 3, 7-O-dimethylquercetin and 70 microM of 3, 7-O-dimethylkaempferol from 100 microM of corresponding substrate were synthesized successfully.
DOI: 10.1007/s10059-010-0098-8
PubMed: 20680484
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Engineering of flavonoid O-methyltransferase for a novel regioselectivity.</title><author><name sortKey="Joe, Eun Ji" sort="Joe, Eun Ji" uniqKey="Joe E" first="Eun Ji" last="Joe">Eun Ji Joe</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Bong Gyu" sort="Kim, Bong Gyu" uniqKey="Kim B" first="Bong-Gyu" last="Kim">Bong-Gyu Kim</name></author><author><name sortKey="An, Byoung Chan" sort="An, Byoung Chan" uniqKey="An B" first="Byoung-Chan" last="An">Byoung-Chan An</name></author><author><name sortKey="Chong, Youhoon" sort="Chong, Youhoon" uniqKey="Chong Y" first="Youhoon" last="Chong">Youhoon Chong</name></author><author><name sortKey="Ahn, Joong Hoon" sort="Ahn, Joong Hoon" uniqKey="Ahn J" first="Joong-Hoon" last="Ahn">Joong-Hoon Ahn</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20680484</idno><idno type="pmid">20680484</idno><idno type="doi">10.1007/s10059-010-0098-8</idno><idno type="wicri:Area/Main/Corpus">003107</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003107</idno><idno type="wicri:Area/Main/Curation">003107</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003107</idno><idno type="wicri:Area/Main/Exploration">003107</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Engineering of flavonoid O-methyltransferase for a novel regioselectivity.</title><author><name sortKey="Joe, Eun Ji" sort="Joe, Eun Ji" uniqKey="Joe E" first="Eun Ji" last="Joe">Eun Ji Joe</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Bong Gyu" sort="Kim, Bong Gyu" uniqKey="Kim B" first="Bong-Gyu" last="Kim">Bong-Gyu Kim</name></author><author><name sortKey="An, Byoung Chan" sort="An, Byoung Chan" uniqKey="An B" first="Byoung-Chan" last="An">Byoung-Chan An</name></author><author><name sortKey="Chong, Youhoon" sort="Chong, Youhoon" uniqKey="Chong Y" first="Youhoon" last="Chong">Youhoon Chong</name></author><author><name sortKey="Ahn, Joong Hoon" sort="Ahn, Joong Hoon" uniqKey="Ahn J" first="Joong-Hoon" last="Ahn">Joong-Hoon Ahn</name></author></analytic><series><title level="j">Molecules and cells</title><idno type="eISSN">0219-1032</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites (MeSH)</term><term>Biotransformation (MeSH)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Escherichia coli (metabolism)</term><term>Flavonoids (metabolism)</term><term>Kaempferols (analysis)</term><term>Kaempferols (chemistry)</term><term>Methyltransferases (chemistry)</term><term>Methyltransferases (metabolism)</term><term>Models, Molecular (MeSH)</term><term>Mutant Proteins (chemistry)</term><term>Mutant Proteins (metabolism)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (enzymology)</term><term>Protein Engineering (methods)</term><term>Quercetin (analysis)</term><term>Quercetin (chemistry)</term><term>Recombinant Proteins (isolation & purification)</term><term>Stereoisomerism (MeSH)</term><term>Substrate Specificity (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biotransformation (MeSH)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Escherichia coli (métabolisme)</term><term>Flavonoïdes (métabolisme)</term><term>Ingénierie des protéines (méthodes)</term><term>Kaempférols (analyse)</term><term>Kaempférols (composition chimique)</term><term>Methyltransferases (composition chimique)</term><term>Methyltransferases (métabolisme)</term><term>Modèles moléculaires (MeSH)</term><term>Populus (enzymologie)</term><term>Protéines mutantes (composition chimique)</term><term>Protéines mutantes (métabolisme)</term><term>Protéines recombinantes (isolement et purification)</term><term>Quercétine (analyse)</term><term>Quercétine (composition chimique)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Sites de fixation (MeSH)</term><term>Spécificité du substrat (MeSH)</term><term>Stéréoisomérie (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Kaempferols</term><term>Quercetin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Kaempferols</term><term>Methyltransferases</term><term>Mutant Proteins</term><term>Quercetin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Flavonoids</term><term>Methyltransferases</term><term>Mutant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Kaempférols</term><term>Quercétine</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Kaempférols</term><term>Methyltransferases</term><term>Protéines mutantes</term><term>Quercétine</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Engineering</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Flavonoïdes</term><term>Methyltransferases</term><term>Protéines mutantes</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Ingénierie des protéines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Biotransformation</term><term>Chromatography, High Pressure Liquid</term><term>Models, Molecular</term><term>Polymerase Chain Reaction</term><term>Stereoisomerism</term><term>Substrate Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biotransformation</term><term>Chromatographie en phase liquide à haute performance</term><term>Modèles moléculaires</term><term>Réaction de polymérisation en chaîne</term><term>Sites de fixation</term><term>Spécificité du substrat</term><term>Stéréoisomérie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An O-methyltransferase isolated from poplar, POMT7, was identified as a flavone 7-O-methyltransferase. In order to generate a mutant of POMT-7 having a novel regioselectivity, we conducted an error-prone polymerase chain reaction. More than 100 mutants were screened and one of the mutants (POMT-M1) Asp257Gly, methylated the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The mutation changed asparagine residue at the position of 257 into glycine. The kinetic parameters showed that the wild type POMT7 was better activity toward kaempferol and quercetin than the POMT7-M1. Using E. coli transformant expressing POMT7-M1, 58 microM of 3, 7-O-dimethylquercetin and 70 microM of 3, 7-O-dimethylkaempferol from 100 microM of corresponding substrate were synthesized successfully.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20680484</PMID><DateCompleted><Year>2010</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">0219-1032</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><Issue>2</Issue><PubDate><Year>2010</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Molecules and cells</Title><ISOAbbreviation>Mol Cells</ISOAbbreviation></Journal><ArticleTitle>Engineering of flavonoid O-methyltransferase for a novel regioselectivity.</ArticleTitle><Pagination><MedlinePgn>137-41</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10059-010-0098-8</ELocationID><Abstract><AbstractText>An O-methyltransferase isolated from poplar, POMT7, was identified as a flavone 7-O-methyltransferase. In order to generate a mutant of POMT-7 having a novel regioselectivity, we conducted an error-prone polymerase chain reaction. More than 100 mutants were screened and one of the mutants (POMT-M1) Asp257Gly, methylated the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The mutation changed asparagine residue at the position of 257 into glycine. The kinetic parameters showed that the wild type POMT7 was better activity toward kaempferol and quercetin than the POMT7-M1. Using E. coli transformant expressing POMT7-M1, 58 microM of 3, 7-O-dimethylquercetin and 70 microM of 3, 7-O-dimethylkaempferol from 100 microM of corresponding substrate were synthesized successfully.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Joe</LastName><ForeName>Eun Ji</ForeName><Initials>EJ</Initials><AffiliationInfo><Affiliation>Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Bong-Gyu</ForeName><Initials>BG</Initials></Author><Author ValidYN="Y"><LastName>An</LastName><ForeName>Byoung-Chan</ForeName><Initials>BC</Initials></Author><Author ValidYN="Y"><LastName>Chong</LastName><ForeName>Youhoon</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Ahn</LastName><ForeName>Joong-Hoon</ForeName><Initials>JH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>07</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>Mol Cells</MedlineTA><NlmUniqueID>9610936</NlmUniqueID><ISSNLinking>1016-8478</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005419">Flavonoids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D044949">Kaempferols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D050505">Mutant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>731P2LE49E</RegistryNumber><NameOfSubstance UI="C006552">kaempferol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9IKM0I5T1E</RegistryNumber><NameOfSubstance UI="D011794">Quercetin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D008780">Methyltransferases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001711" MajorTopicYN="N">Biotransformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005419" MajorTopicYN="N">Flavonoids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044949" MajorTopicYN="N">Kaempferols</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008780" MajorTopicYN="N">Methyltransferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050505" MajorTopicYN="N">Mutant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015202" MajorTopicYN="N">Protein Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011794" MajorTopicYN="N">Quercetin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013237" MajorTopicYN="N">Stereoisomerism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>04</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>04</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>8</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>8</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20680484</ArticleId><ArticleId IdType="doi">10.1007/s10059-010-0098-8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><noCountry><name sortKey="Ahn, Joong Hoon" sort="Ahn, Joong Hoon" uniqKey="Ahn J" first="Joong-Hoon" last="Ahn">Joong-Hoon Ahn</name><name sortKey="An, Byoung Chan" sort="An, Byoung Chan" uniqKey="An B" first="Byoung-Chan" last="An">Byoung-Chan An</name><name sortKey="Chong, Youhoon" sort="Chong, Youhoon" uniqKey="Chong Y" first="Youhoon" last="Chong">Youhoon Chong</name><name sortKey="Kim, Bong Gyu" sort="Kim, Bong Gyu" uniqKey="Kim B" first="Bong-Gyu" last="Kim">Bong-Gyu Kim</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Joe, Eun Ji" sort="Joe, Eun Ji" uniqKey="Joe E" first="Eun Ji" last="Joe">Eun Ji Joe</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003277 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003277 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20680484
|texte= Engineering of flavonoid O-methyltransferase for a novel regioselectivity.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20680484" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |